novel human prostate epithelial cell culture models for the study of carcinogenesis and of normal stem cells and cancer stem cells
Báo cáo khoa học: " Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection" ppsx
... physiologically relevant system for further in vitro studies of HCV entry and infection dynamics Methods Cell culture and viruses Huh7 cells (also known as Huh7/scr cells [25,26] and Huh7-1 cells [27]) were ... Establishment of Huh7 3-D Aggregates To assess the utility of the RWV as a culture method for Huh7 cells, Huh7 cells were cultured on Cytodex-3 microcarrier beads in the RWV for 26 days Morphological and ... 4B shows that the majority of Huh7 cells were positive for the HCV E2 glycoprotein and that the entire aggregate was permissive for HCV infection rather than just the cells at the periphery,...
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Báo cáo khoa học: "Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection" pdf
... physiologically relevant system for further in vitro studies of HCV entry and infection dynamics Methods Cell culture and viruses Huh7 cells (also known as Huh7/scr cells [25,26] and Huh7-1 cells [27]) were ... Establishment of Huh7 3-D Aggregates To assess the utility of the RWV as a culture method for Huh7 cells, Huh7 cells were cultured on Cytodex-3 microcarrier beads in the RWV for 26 days Morphological and ... 4B shows that the majority of Huh7 cells were positive for the HCV E2 glycoprotein and that the entire aggregate was permissive for HCV infection rather than just the cells at the periphery,...
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Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot
... aspirating the medium, washing the cells with PBS and adding the nanoparticle-containing medium After the exposure time, the cells were analyzed microscopically The total number of cells and the number ... penetrate the cell membrane and therefore stains membrane-damaged cells only Cell viability of A549 cells was determined by culturing the cells for 48 h on the Si3N4 membrane at 37°C and 5% CO2 ... in the corner areas of the microcavity (Figure 3f) The performed studies verify the biocompatibility of the Si3N4 membrane and the suitability of the microcavity for in vitro studies A549 cells...
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Báo cáo y học: " The utilization of humanized mouse models for the study of human retroviral infections" docx
... tissues, therefore allowing the engraftment of human cells and/ or tissues This model represents the landmark experiment that sparked further development of humanized mice for the study of human ... Additionally, the presence of the fetal thy/ liv organ allows for innate maturation of human CD4+ and CD8+ T cells in the context of the animal's own immune system In general, the proliferation of human cells ... robust systemic reconstitution of the mouse with human cells is possible due to the education of human T cells within the engrafted thymus, as well as the maturation of human hematopoietic cells...
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báo cáo hóa học: " Muscle-driven forward dynamic simulations for the study of normal and pathological gait" potx
... model; the body segment inertial parameters; kinematic behavior of the joints; the bony geometry and muscle attachment locations; the mathematical model of muscle force generation; the muscle force ... function such as the aggregate deviation from a given motion Each of these choices has the potential to affect the performance of the simulation and thus may also affect the validity of clinical applications ... both the force-length and force-velocity properties This model performed better than did models with muscles lacking either of these properties or a model actuated by moments rather than muscle forces...
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Báo cáo y học: "A novel human ex vivo model for the analysis of molecular events during lung cancer chemotherapy" pptx
... out the LIFE/DEAD-assay and 2-photon microscopy PZ was involved in the design of the study, evaluation of the results and covered the clinical part EV was involved in the design of the study and ... the study and was involved in the evaluation of the data as well as the writing of the manuscript All authors read and approved the final manuscript The chemo-sensitivity of SCLC and breast cancer ... viability and functionality of the cells Furthermore, specimens of breast cancer were also treated like the lung tumor samples to verify the efficiency of the used drug concentrations on the chosen...
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báo cáo hóa học:" Identifying alemtuzumab as an anti-myeloid cell antiangiogenic therapy for the treatment of ovarian cancer" pdf
... critical role for these cells in tumorigenesis and indicate that these cells may be novel therapeutic targets for solid tumor therapy Genetic manipulations to inhibit or eliminate these cells in both ... myeloid cells appear to be a primary source of resistance to antiVEGF therapy, suggesting a critical role for these cells in tumor angiogenesis [5] The exact mechanism of action of myeloid cells ... we have termed these cells vascular leukocytes (VLC) [15,21] VLC represent 10–70% of host cells and up to 30% of all cells in ovarian cancer ([21] and unpublished data In vitro and in vivo studies...
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Báo cáo sinh học: "On the relevance of three genetic models for the description of genetic variance in small populations undergoing selection" docx
... the simulation The analytical models of Verrier et al [14] and Chevalet [3] will then be described, each corresponding to a different choice of hypotheses for the algebraic formalization of the ... with the theory, of the relationships between mates and inbreeding induced in the offspring and of the changing variance of gene effects due to genetic drift and faster or slower fixation of alleles ... that under the hypothesis of a very large number of independent identical loci, the oligogenic model and the Gaussian theory agree closely These first results validate the use of the ’full-finite’...
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báo cáo khoa học: "Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice" pptx
... understood Cell fusion, in which stem cells fuse with the somatic cell in the niche, had been suggested by many authors [11] and strong evidences were reported for the transdifferentiation of stem cells ... stem cells at the target site was monitored by the expression of sry gene after transplantation of stem cells in sex mismatched mice The expression of sry gene confirmed the engraftment of both ... kinetics, distribution and stability of HGFCNP in the blood circulation Whether HGF released from the CNP causes the stem cell differentiation or apoptosis of myofibroflasts or both of these functions...
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Báo cáo y học: "Use of a multi-virus array for the study of human viral and retroviral pathogens: gene expression studies and ChIP-chip analysis" pot
... Table listing the identity of the PCR primers used for the production of the amplicons, their exact coordinates on the genomes, the sizes of the probes generated and the success of the amplification ... ChIP of uninduced BCBL-1 cells BCBL-1 cells were cultured normally and a ChIP was performed as described in Materials and Methods All amplicons are listed at the bottom of the panel (B) ChIP of ... data and printed the arrays NM printed the arrays and participated in some of the expression studies AP performed many of the expression studies and the ChIP-chip analysis VL performed the ChIP-chip...
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Tài liệu Towards a framework for the study of the neural correlates of aesthetic preference pdf
... of reward value and the awareness of the emotional state The cortical processing of the magnitude of the reward of the stimuli to be aesthetically judged probably corresponds to activity in the ... division The latter encompasses the region identified by Vartanian and Goel (2004), and has been related with the assessment of the relevance of motivational and emotional information and the regulation ... the complexity, novelty, color spectrum and luminance of the stimuli, in addition to their size and resolution, Vartanian and Goel (2004) and Kawabata and Zeki (2004) did not report whether they...
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Report Of The Committee On Proposal Evaluation For Allocation Of Supercomputing Time For The Study Of Molecular Dynamics pptx
... letter describes the work and transmits the final report of the Committee on Proposal Evaluation for Allocation of Supercomputing Time for the Study of Molecular Dynamics, Second Round The committee ... of experts to evaluate the proposals submitted in response to the aforementioned RFP The committee of 17 was chaired by Dr Robert L Jernigan, Director of the Baker Center for Bioinformatics and ... the areas of protein-lipid interactions and channel functions and who provided the committee with an initial assessment of a subset of the proposal submissions The goal of the second RFP for Biomolecular...
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Báo cáo khoa học: Data-driven docking for the study of biomolecular complexes pptx
... depends on the kind of data used (e.g the level of detail and the amount of inherent ambiguity) and the sampling method ‘Geometric’ methods might limit the number of orientations selected for docking ... In the docking literature one often finds the distinction between ‘bound’ and ‘unbound’ docking: the former refers to docking using the structures of the single proteins as they are present in the ... assessment of which residues of the labeled molecule are perturbed by the formation of the complex One then repeats this procedure with the second molecule labeled Under the assumption that the perturbed...
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A FUNCTIONAL-ANALYTIC METHOD FOR THE STUDY OF DIFFERENCE EQUATIONS EUGENIA N. PETROPOULOU AND potx
... equations of three and four variables The aim of this paper is to present the generalization of this functional-analytic method for the study of linear and nonlinear partial difference equations of p ... see [11] and the references therein) Also, by assuring the existence of a solution of a difference equation in the space or , we obtain information regarding the asymptotic behavior of the unknown ... fixed point theorems) from the wealth of operator theory, in order to assure the existence of a unique solution of the operator equation in H or H1 In the case of linear equations, we use the following...
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Development of a fluorescence correlation spectroscopy method for the study of biomolecular interactions
... action: ratio of the concentrations of the free receptor [R] and the free ligand [L], to the concentration of the complex [RL] The concentrations of the free components are then given by the total ... Chapter Theory and Setup Here, Q is the product of the absorption coefficient and the molecular quantum yield of the fluorescent species κ is the detection efficiency of the instrument including the detector ... constant k, the absolute temperature T and the friction coefficient f For the case of a spherical molecule, f = 6πηr (2.20) where η is the viscosity of the solvent, and r is the radius of the sphere...
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An asthma allergen specific animal model for the study of responses to dust mite allergen induced asthma
... found on DCs and bronchial epithelial cells and the presence of TSLP can induce DC activation and the production of IL-13 from epithelial cells TSLP also promote the development of basophils, ... orchestrator of immune responses These cells produce a variety of cytokines that affect the function of the other cells in the immune system, both in the adaptive and innate immune components of the immune ... components: the cell- mediated and the humoral immune response CD4 and CD8 T cells are involved in the cell- mediated responses while B cells are the key players in the humoral response The ability of the...
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Inverse modeling for the study of 2d doping profile of submicron transistor using process and device simulation
... is the range of the amorphous Pearson profile Rpb is the range of the channeled Pearson profile σ a is the standard deviation of the amorphous Pearson profile σ b is the standard deviation of the ... channeled Pearson profile γ a is the skewness of the amorphous Pearson profile γ b is the skewness of the channeled Pearson profile β a is the kurtosis of the amorphous Pearson profile - 28 - b β ... Ichanneled are the normalized channeled and amorphous Pearson profiles, respectively, ratio is the ratio of the dose of the amorphous profile to the total dose and u is the coordinate along the depth...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... certain human cell types under hypoxia Experimental procedures Cell culture and hypoxia study The human cell lines used were KG1 myeloid cells, K562 erythroid cells, and Jurkat T-lymphocyte cells and ... oxygen) and returned to the basal level after 14 days of hypoxia [26] It is therefore of significance to study the regulation of HO-2 expression in human cells under hypoxia In the present study, ... mRNA in YN-1 cells B Fig Decreased expression of heme oxygenase (HO)-1 and HO-2 under hypoxia in human cancer cells (A) Northern blot analysis of HO-1 and HO-2 mRNA HeLa cervical cancer and HepG2...
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epithelial cell culture protocols
... including smooth muscle cells, mesenchymal cells, vessel forming cells, nerve cells, blood cells, including immune cells and gastric gland cells Gastric epithelial cells can be further subdivided into ... protocol, and thereafter, modified methods for the culture of fetal mouse epithelial cells and of human epithelial cells from children are given Materials 2.1 Culture of Newborn Mouse Thymic Epithelial ... Cell Culture 1 Human Lens Epithelial Cell Culture Nobuhiro Ibaraki Introduction Crystalline lens consists of epithelial cells, fiber cells, and a capsule They originate from lens epithelial cells...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
... stimulation of PBMCs was shown to elicit absolute expansion of NK cells and CD56+ T cells, e.g NKT cells, γδ T cells and some αβ/CD8+ T cells [30] The combination of IL-2 and 3a-G1 in our hands also ... slight increase in the percentage of apoptotic cells was observed when cells were cultured with 3a-G1 but most of the cells maintained their DNA integrity Conversely, the proportion of mitotic events ... presence of few remaining regulatory T cells could have a detrimental effect for the activity of infused NK cells in vivo, it does not affect the cytotoxic property of the expanded NK cells in...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25